Effect of different organic salts on amino acids partition behaviour in PEG-salt ATPS

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Eugénia A. Macedo (Author) FEUP; Kamila Wysoczanska (Author) Other; Hoang Tam Do (Author) Other; Christoph Held (Author) Other; Gabriele Sadowski (Author) Other

Journal

Title: Fluid Phase Equilibria

Vol. 456

Pages: 84-91

ISSN: 0378-3812

Publisher: Elsevier

Indexing

ISI Web of Science

Scopus

INSPEC

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Associated Projects

AIProcMat@N2020 - Advanced Industrial Processes and Materials for a Sustainable North Region of Portugal2020

Other information

DOI: 10.1016/j.fluid.2017.10.007

Abstract (EN): The phase diagrams of six different polyethylene glycol (PEG)esalt aqueous two-phase systems (ATPS) were measured at T ¼ 298.15 K and p ¼ 1 bar. PEG of different molecular weight (4000, 6000 and 8000) and organic salts (potassium citrate, potassium sodium tartrate) were used to form the biphasic systems. The results were compared with data for PEGesodium citrate ATPS, reported in the literature (PEG 4000, PEG 6000) and measured in this work (PEG 8000). The partition of four dinitrophenyl-amino acids was measured in these ATPS for different tie-line lengths. Based on these data, cation and anion effects were evaluated in terms of the relative hydrophobicity of the phases using DG*(CH2) calculations. The distribution coefficients have been obtained spectrophotometrically. Studies on the partitioning indicate the advantage of citrate salts over tartrate salts as well as sodium-based salts over potassium-based salts. This consistently results from the (liquid-liquid) phase behaviour of these systems.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8