DNA barcoding coupled to HRM analysis as a new and simple tool for the authentication of Gadidae fish species

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Fernandes, TJR (Author) Other; Joana Costa (Author) FFUP; Beatriz B P P Oliveira (Author) FFUP; Mafra, I (Author) Other

Journal

Title: Food Chemistry

Vol. 230

Pages: 49-57

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 35 Citations

Other information

Authenticus ID: P-00M-HK9

DOI: 10.1016/j.foodchem.2017.03.015

Abstract (EN): This work aimed to exploit the use of DNA mini-barcodes combined with high resolution melting (HRM) for the authentication of gadoid species: Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Theragra chalcogramma) and saithe (Pollachius virens). Two DNA barcode regions, namely cytochrome c oxidase subunit I (COI) and cytochrome b (cytb), were analysed in silico to identify genetic variability among the four species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis. The cytb mini-barcode enabled best discrimination of the target species with a high level of confidence (99.3%). The approach was applied successfully to identify gadoid species in 30 fish containing foods, 30% of which were not as declared on the label. Herein, a novel approach for rapid, simple and cost-effective discrimination/clustering, as a tool to authenticate Gadidae fish species, according to their genetic relationship, is proposed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9