Novel quantitative real-time PCR approach to determine safflower (Carthamus tinctorius) adulteration in saffron (Crocus sativus)

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Villa, C (Author) Other; Joana Costa (Author) FFUP; Beatriz B P P Oliveira (Author) FFUP; Mafra, I (Author) Other

Journal

Title: Food Chemistry

Vol. 229

Pages: 680-687

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 26 Citations

Other information

Authenticus ID: P-00M-GV5

DOI: 10.1016/j.foodchem.2017.02.136

Abstract (EN): This work intended to develop DNA-based methods to detect and quantify safflower as an adulterant in saffron. Species-specific PCR and real-time PCR with EvaGreen dye targeting the ITS region of Carthamus tinctorius L. (safflower) were successfully proposed. The assays allowed absolute and relative sensitivities of 2 pg of safflower DNA (similar to 1.4 DNA copies) and 0.1% of safflower in saffron (Crocus sativus L.), respectively. A normalised real-time PCR approach was also proposed in the range of 0.1-20% (w/w) of safflower in saffron, which was successfully validated and applied to commercial saffron samples (stigmas, powders and seasonings). From 19 samples, three were positive to safflower, though at levels below the limit of detection, suggesting cross-contamination rather than adulteration. In this work, specific, sensitive and accurate tools were proposed to authenticate saffron. To the best of our knowledge, this is the first successful attempt to quantify safflower by a DNA-based approach.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8