COI barcode-HRM as a novel approach for the discrimination of hake species

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Fernandes, TJR (Author) Other; Joana Costa (Author) FFUP; Beatriz B P P Oliveira (Author) FFUP; Mafra, I (Author) Other

Journal

Title: Fisheries Research

Vol. 197

Pages: 50-59

ISSN: 0165-7836

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-00N-5GV

DOI: 10.1016/j.fishres.2017.09.014

Abstract (EN): Hake species of Merluccius genus represent an important group of fish commonly sold all over the world. Therefore, they are highly prone to be adulterated, particularly the substitution of M. merluccius by other species with lower market value. The present work intended the development of a highly sensitive methodology for the rapid detection and differentiation of hake species based on mini-barcoding of cytochrome c oxidase subunit I (COI) gene combined with high resolution melting (HRM) analysis. The method allowed the full discrimination of M. merluccius, M. productus, M. hubbsi, M. capensis and M. paradoxus with high levels of confidence. Real-time PCR assay targeting COI mini-barcode provided a high sensitive tool to detect hake species down to 0.2-20 pg of DNA with adequate performance parameters. The application of the COI-HRM approach to 45 fish-containing foods showed that two samples did not comply with the declared species, suggesting mislabelling or species substitution. These findings highlight the need of controlling processed fish-containing foods and the feasibility of the proposed tool for their authentication at trace levels.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10