Autolysis of intracellular content of Brewer's spent yeast to maximize ACE-inhibitory and antioxidant activities

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Vieira, EF (Author) Other; Melo, A (Author) Other; Ferreira, Isabel (Author) FFUP

Journal

Title: LWT - Food Science and Technology

Vol. 82

Pages: 255-259

ISSN: 0023-6438

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00Z-HB8

DOI: 10.1016/j.lwt.2017.04.046

Abstract (EN): Brewer's spent yeast (BSY) is the second major by-product from brewing and is recognized as a promising source of potentially bioactive ingredients, namely the cell-wall ß-glucans. However, to make the process of ß-glucans¿ separation from BSY profitable, new applications for the inner cell content are required. Mechanical disruption of BSY cell wall was performed to separate the ß-glucans rich fraction and obtain an extract from intracellular content, rich in native proteins and enzymes, which was submitted to further autolysis. This short communication describes the optimization of BSY extract autolysis, by Response surface methodology (RSM), to produce a BSY autolysate comprising antioxidant and ACE-inhibitory activities. RSM enabled the prediction of optimum autolysis conditions. Autolysis of BSY inner cell content at 36.0 °C, 6.0 h gave a TPC of 385 ¿M GAE/mL, a FRAP value of 374 ¿M TE/mL and an IC50 value of 379 ¿g/mL. These experimental results were in agreement with the RSM predicted values. The combination of ACE-I and antioxidant activities in one autolysate can be very useful as functional ingredient for food industry and complement the extractive process of ß-glucans from cell wall. © 2017

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5