Impact of in Vitro Gastrointestinal Digestion and Transepithelial Transport on Antioxidant and ACE-Inhibitory Activities of Brewer's Spent Yeast Autolysate

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Vieira, EF (Author) Other; das Neves, J (Author) Other; Vitorino, R (Author) FMUP; da Silva, DD (Author) Other; Helena Carmo (Author) FFUP; Ferreira, Isabel (Author) FFUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 64

Pages: 7335-7341

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 25 Citations

Other information

Authenticus ID: P-00M-4S4

DOI: 10.1021/acs.jafc.6b02719

Abstract (EN): Brewer's spent yeast (BSY) autolysates may have potential applications as food ingredients or nutraceuticals due to their antioxidant and ACE-inhibitory activities. The impact of simulated gastrointestinal (GI) digestion, the interaction with intracellular sources of oxidative stress, the intestinal cell permeability of BSY peptides, and the antioxidant and ACE-inhibitory activities of BSY permeates were assayed. Gastrointestinal digestion of BSY autolysates enhanced antioxidant and ACE-inhibitory activities as measured in vitro. No cytotoxic effects were observed on Caco-2 cells after exposure to the digested BSY autolysates within a concentration range of 0.5 to 3.0 mg of peptides/mL. A protective role to induced oxidative stress was observed. The transepithelial transport assays indicate high apparent permeability coefficient (P-app) values for BSY peptides across Caco-2/HT29-MTX cell monolayer (14.5-26.1 X 10(-6) cm/s) and for Caco-2 cell monolayer model (12.4-20.8 X 10(-6) cm/s), while the antioxidant and ACE-inhibitory activities found in flux material from the basolateral side suggest transepithelial absorption of bioactive compounds.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7