Brain-targeted delivery of resveratrol using solid lipid nanoparticles functionalized with apolipoprotein E

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Neves, AR (Author) Other; Queiroz, JF (Author) Other; Salette Reis (Author) FFUP

Journal

Title: Journal of Nanobiotechnology

Vol. 14

ISSN: 1477-3155

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 42 Citations

Scopus - 91 Citations

Other information

Authenticus ID: P-00K-CQF

DOI: 10.1186/s12951-016-0177-x

Abstract (EN): Background: The present study takes advantage of the beneficial effects of resveratrol as a neuroprotective compound. Resveratrol-loaded solid lipid nanoparticles were functionalized with apolipoprotein E which can be recognized by the LDL receptors overexpressed on the blood-brain barrier. Results: Transmission electron microscopy images revealed spherical nanoparticles, dynamic light scattering gave a Z-average lower than 200 nm, and a zeta potential of around - 13 mV and very high resveratrol entrapment efficiency (ca. 90 %). In vitro cytotoxic effects were assessed by MTT and LDH assays in hCMEC/D3 cell line and revealed no toxicity up to 50 mu M over 4 h of incubation. The permeability through hCMEC/D3 monolayers showed a significant increase (1.8-fold higher) for resveratrol-loaded solid lipid nanoparticles functionalized with apolipoprotein E when compared to non-functionalized ones. Conclusions: In conclusion, these nanosystems might be a promising strategy for resveratrol delivery into the brain, while protecting it from degradation in the blood stream.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11