pH-sensitive nanoparticles for improved oral delivery of dapsone: risk assessment, design, optimization and characterization

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Chaves, LL (Author) Other; Costa Lima, SAC (Author) Other; Vieira, ACC (Author) Other; Barreiros, L (Author) Other; Marcela A Segundo (Author) FFUP; Ferreira, DC (Author) FFUP; Sarmento, B (Author) Other; Salette Reis (Author) FFUP

Journal

Title: Nanomedicine

Vol. 12

Pages: 1975-1990

ISSN: 1743-5889

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00N-G7H

DOI: 10.2217/nnm-2017-0105

Abstract (EN): Aim: To optimize the production of pH-sensitive dapsone (DAP) nanoparticles based on Eugradit L100 (NPs-EL100-DAP) for oral delivery. Materials & methods: NPs-EL100-DAP were optimized using a Plackett-Burman design and a Box-Behnken design. The physicochemical properties of the obtained nanoparticles were monitored by microscopy, dynamic light scattering, Fourier transform infrared spectroscopy, differential scanning calorimetry, in vitro release assays, and examined for cytotoxicity and permeation across intestinal barrier. Results: The in vitro release assay of NPs-EL100-DAP confirmed the nanoparticles' pH sensitivity and the ability to deliver DAP at intestinal environment. NPs-EL100-DAP demonstrated enhanced intestinal interactions in comparison to free DAP, across Caco-2 monolayers. Conclusion: These studies demonstrate the potential of NPs-EL100-DAP as a therapeutic platform for oral treatment of leprosy.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16