Apo E-Functionalization of Solid Lipid Nanoparticles Enhances Brain Drug Delivery: Uptake Mechanism and Transport Pathways

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Neves, AR (Author) Other; Queiroz, JF (Author) Other; Lima, SAC (Author) Other; Salette Reis (Author) FFUP

Journal

Title: Bioconjugate Chemistry

Vol. 28

Pages: 995-1004

ISSN: 1043-1802

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 41 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00M-Q6W

DOI: 10.1021/acs.bioconjchem.6b00705

Abstract (EN): Several strategies have been implemented to enhance brain drug delivery, and herein solid lipid nanoparticles functionalized with apolipoprotein E were tested in hCMEC/D3 cell monolayers. The mean diameter of 160 nm, negative charge of -12 mV, and their lipophilic characteristics make these nanosystems suitable for brain delivery. Confocal images and flow cytometry data showed a cellular uptake increase of 1.8-fold for SLN-Palmitate-ApoE and 1.9-fold for SLN-DSPE-ApoE when compared with the non-functionalized SLNs. Clathrin-mediated endocytosis was distinguished as the preferential internalization pathway involved in cellular uptake and nanoparticles could cross the blood-brain barrier predominantly by a transcellular pathway. The understanding of the mechanisms involved in the transport of these nanosystems through the blood brain barrier may potentiate their application on brain drug delivery.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10