Imported poultry meat as a source of extended-spectrum cephalosporin-resistant CMY-2-producing Salmonella Heidelberg and Salmonella Minnesota in the European Union, 2014-2015

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Campos, J (Author) Other; Mourao, J (Author) Other; Silveira, L (Author) Other; Saraiva, M (Author) Other; Correia, CB (Author) Other; Macas, AP (Author) Other; Luisa Peixe (Author) FFUP; Antunes, Patrícia (Author) FCNAUP

Journal

Title: International Journal of Antimicrobial Agents

Vol. 51

Pages: 151-154

ISSN: 0924-8579

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 39 Citations

Scopus - 37 Citations

Scientific classification

CORDIS: Health sciences

FOS: Medical and Health sciences

Associated Projects

FOODnanoHEALTH - FOODnanoHEALTH

Other information

Authenticus ID: P-00N-FHN

DOI: 10.1016/j.ijantimicag.2017.09.006

Abstract (EN): Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of poultry meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from poultry meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and beta-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum beta-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4