Unravelling the genome of a Pseudomonas aeruginosa isolate belonging to the high-risk clone ST235 reveals an integrative conjugative element housing a bla(GES-6) carbapenemase

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Botelho, J (Author) Other; Grosso, F (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: Journal of Antimicrobial Chemotherapy

Vol. 73

Pages: 77-83

ISSN: 0305-7453

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 15 Citations

Other information

Authenticus ID: P-00N-FHA

DOI: 10.1093/jac/dkx337

Abstract (EN): Objectives: In Pseudomonas aeruginosa, the bla(GES-6) carbapenemase gene was previously associated with an In1076 class I integron. Here, we conducted a genome-based analysis and explored the genetic platform associated with the mobility of this gene. Methods: WGS of a bla(GES-6)-harbouring P. aeruginosa isolate (FFUP_PS_690) was performed with Illumina HiSeq, de novo assembly was performed using SPAdes and subsequent bioinformatic analysis was performed concerning antibiotic resistance genes, virulence features and mobile genetic elements. Results: The FFUP_PS_690 isolate belongs to the ST235 high-risk clone and houses a novel integrative conjugative element (ICE), hereby named ICEPae690. This clc-like ICE comprises the bla(GES-6)-harbouring In1076 integron and specific modules. An ExoU island A variant was also identified. Conclusions: The presence of a 'hitch-hiking' bla(GES-6)-harbouring In1076 integron in an ICE and an exoU-carrying genomic island highlight the potential spread of these elements through conjugation and/or clonal expansion of the ST235 lineage.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7