Hydrogen peroxide-induced secondary necrosis in conidia of Aspergillus fumigatus

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Oliveira, M (Author) FCUP; Pereira, C (Author) Other; Bessa, C (Author) Other; Araujo, R (Author) Other; Lucilia Saraiva (Author) FFUP

Journal

Title: Canadian Journal of Microbiology

Vol. 62

Pages: 95-101

ISSN: 0008-4166

Publisher: NRC Research Press

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Other information

Authenticus ID: P-00K-54B

DOI: 10.1139/cjm-2015-0561

Abstract (EN): Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell death. Additionally, the mechanism of H2O2-induced cell death was studied in A. fumigatus conidia for the first time. Data presented in this work reveal that the H2O2-induced conidial cell death was associated with a marked increase of TUNEL-and PI-positive cells. It is therefore suggested that conidia cell death occurs in a dose-dependent manner through a secondary necrosis mechanism. The knowledge of conidia cell death machinery may provide insights into the molecular mechanism of conidia-mediated toxicity to the respiratory tract and may pave the way for improved therapeutic approaches against A. fumigatus conidia-mediated diseases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7