Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Dias, TR (Author) Other; Alves, MG (Author) Other; Silva, J (Author) Other; barros, a (Author) FMUP; sousa, m (Author) ICBAS; Susana Casal (Author) FFUP; Silva, BM (Author) Other; Oliveira, PF (Author) FMUP

Journal

Title: Toxicology in Vitro

Vol. 41

Pages: 214-222

ISSN: 0887-2333

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 15 Citations

Other information

Authenticus ID: P-00M-KMV

DOI: 10.1016/j.tiv.2017.03.006

Abstract (EN): Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50 mu M, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50 mu M) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50 mu M) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis. (C) 2017 Published by Elsevier Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9