Activation of ionic channels by deoxycholate in frog and human cell lines

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Mauricio, AC (Author) ICBAS; Ferreira, KTG (Author) Other

Journal

Title: Experimental Physiology

Vol. 84

Pages: 489-499

ISSN: 0958-0670

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-001-4D1

DOI: 10.1017/s0958067099017340

Abstract (EN): Humans, after extensive ileal resection, frequently suffer from diarrhoea, which may be due to an increased delivery of deoxycholate (DOC) to the large intestine. In the frog skin the addition of DOC (0.5 mM) to the apical side induced the activation of amiloride-sensitive Na+ channels and an increase in the unidirectional Cl- fluxes. Here we used two established cell lines (A(6) and Caco(2)) to study the effect of DOC on ion channels at cell and membrane level using the patch-clamp technique. In A(6) cells subcultured directly on Petri dishes and studied in the whole-cell configuration, DOC induced an increase in cell conductance of 110.3 +/- 4 pS pF(-1) (N = 8) which was reduced to 89 +/- 14 pS pF(-1) (N = 8) by the addition of DIDS (0.5 mM). The absolute values of these two effects were not statistically different (P < 0.2). In Caco(2) cells, the addition of DOC (0.5 mM) induced, after 1 min, an increase in cell conductance of 583 +/- 16 pS pF(-1) (N = 8) which was reduced to 560.4 +/- 16 pS pF(-1) (N = 8) by DIDS (0.5 mM) and N-phenylanthranilic acid (DPC; 0.5 mM). The two values were not statistically different (P < 0.4). In Caco(2) cells subcultured under the same conditions, DOC induced an increase in cell conductance of 1710 +/- 64 pS pF(-1) (N = 6). Subsequent addition of amiloride (0.1 mM) reduced the cell conductance to 1558 +/- 33 pS pF(-1) (N = 6). These two mean values were statistically different allowing for an error of the second kind < 0.05. In cells in which DOC produced a conductance increase of 1010 +/- 10 pS pF(-1), gadulinium (0.5 mM) induced a fall in cell conductance of 1800 +/- 10 pS pF(-1). In Caco(2) cells, addition of DOC (0.5 mM) to the bath reversibly induced the appearance of or an increase in channel activity in patches studied in cell-attached and excised inside-out configuration. In inside-out experiments (N = 13) DOC (0.5 mM) induced the appearance of channel activity with conductances and reversal potentials (E-r) of 27.7 +/- 1.9 pS and 0.8 +/- 5.7 mV, respectively. In cell-attached patches (N = 13) these values were 24.9 +/- 4.4 pS and -18.1 +/- 6.4 mV. In excised inside-out patches from Caco, cells, subjected to electrochemical gradients for Na+ K+ and Cl-, (+85, -85 and 0 mV, respectively), addition of DOC also induced an increase in the baseline conductance and a shift in the reversal potential from values around +25 mV to values around 0 mV. Bile salts activated both anionic and cationic channels and did not require the presence of intracellular factors for these effects. We suggest that they act at the membrane level.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11