Studies on the biocompatibility of bacterial cellulose

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Andrade, FK (Author) Other; Alexandre, N (Author) Other; Amorim, I (Author) Other; Gartner, F (Author) Other; Mauricio, AC (Author) ICBAS; Luis, AL (Author) Other; Gama, M (Author) Other

Journal

Title: Journal of Bioactive and Compatible Polymers

Vol. 28

Pages: 97-112

ISSN: 0883-9115

Publisher: SAGE

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 46 Citations

Other information

Authenticus ID: P-002-1VM

DOI: 10.1177/0883911512467643

Abstract (EN): Bacterial cellulose was functionalized with a chimeric protein containing a cellulose-binding module and the adhesion peptide Arg-Gly-Asp. Small-diameter bacterial cellulose membranes were produced and subcutaneously implanted in sheep for 1-32 weeks. The implants triggered a biological response similar to other high surface-to-volume implants. There were no significant differences in the inflammation degree between the bacterial cellulose coated with the recombinant protein Arg-Gly-Asp-cellulose-binding module and the native bacterial cellulose. The implants were considered to be mildly irritating to the tissue compared to the negative control sample (expanded polytetrafluoroethylene). The analysis of the fluorescence microscopy revealed that, apart from increasing cell adhesion, the presence of Arg-Gly-Asp stimulated an even cell distribution, while the cells on the untreated bacterial cellulose seemed to form aggregates. Furthermore, the cells on the Arg-Gly-Asp-treated bacterial cellulose presented a more elongated morphology. Mechanical tests indicated that the small-diameter bacterial cellulose tubes were more elastic than the human arteries and veins.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16