Cytotoxicity of denture adhesives

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Gomes PS (Author) FMDUP; Maria Helena Figueiral (Author) FMDUP; Fernandes MH (Author) FMDUP; Scully, C (Author) Other

Journal

Title: Clinical Oral Investigations

Vol. 15 No. 6

Pages: 885-893

ISSN: 1432-6981

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 27 Citations

Scopus - 29 Citations

Other information

Authenticus ID: P-002-JBX

DOI: 10.1007/s00784-010-0464-0

Abstract (EN): Ten commercially available denture adhesives, nine soluble formulations (six creams, three powders) and one insoluble product (pad), were analyzed regarding the cytotoxicity profile in direct and indirect assays using L929 fibroblast cells. In the direct assay, fibroblasts were seeded over the surface of a thick adhesive gel (5%, creams; 2.5%, powders and pad). In the indirect assay, cells were cultured in the presence of adhesive extracts prepared in static and dynamic conditions (0.5-2%, creams; 0.25-1%, powders and pad). Cell toxicity was assessed for cell viability/proliferation (MTT assay) and cell morphology (observation of the F-actin cytoskeleton organization by confocal laser scanning microscopy). Direct contact of the L929 fibroblasts with the thick adhesive gels caused no, or only a slight, decrease in cell viability/proliferation. The adhesive extracts (especially those prepared in dynamic conditions) caused significantly higher growth inhibition of fibroblasts and, in addition, caused dose- and time-dependent effects, throughout the 6-72 h exposure time. Also, dose-dependent effects on cell morphology, with evident disruption of the F-actin cytoskeleton organization, were seen in the presence of most adhesives. In conclusion, the adhesives possessed different degrees of cytotoxicity, but similar dose- and time-dependent biological profiles.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9