Ontogenic variations in free and esterified fatty acids during somatic embryogenesis of flax (Linum usitatissimum L.)

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Cunha, AC (Author) Other; Fernandes Ferreira, M (Author) Other

Journal

Title: Plant Science

Vol. 164

Pages: 863-872

ISSN: 0168-9452

Publisher: Elsevier

Indexing

Scopus - 9 Citations

Other information

Authenticus ID: P-00F-H08

DOI: 10.1016/s0168-9452(03)00074-8

Abstract (EN): In vitro cultures of flax (Linum usitatissimum L.) were established on MS medium and four samplings were made during the 7 weeks of culture. The samples varied from the original hypocotyl segments (HS) at t0 and segments with incipient calli formation (HSC) after 2 weeks (t2), to embryogenic calli (EC), non-embryogenic calli (NEC) and somatic embryos (SE) collected after 5 (t5) and 7 weeks (t7). The respective free fatty acids (FFA) and esterified fatty acids (EFA) were extracted and analyzed, as methyl esters, by GC and GC-MS, and the data were submitted to analysis of variance (ANOVA) and PCA statistical analysis. The main FFA in all samples was 16:0, 18:0, 18:1, 18:2 and 18:3. EFA fractions were more diverse containing some less common FA. The development of SE was associated with a decrease in neutral lipids. Both the total FFA and the total EFA, as fractions of total lipids, increased with the dedifferentiation and the early calli formation leveling off thereafter (t5-t7). FA variations related to dedifferentiation of hypocotyl tissues and growth of NEC, somatic embryogenesis, and development of SE were observed. Predominant or tissue-specific FA was also found. The value of 18:3/18:2 and 18:0/18:3 ratios as potential indicators of SE autotrophy and calli embryogenic capacity is discussed. © 2003 Published by Elsevier Science Ireland Ltd.

Language: English

Type (Professor's evaluation): Scientific