Anthelmintic, Antibacterial and Cytotoxicity Activity of Imidazole Alkaloids from Pilocarpus microphyllus Leaves

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Rocha, JA (Author) Other; Andrade, IM (Author) Other; Veras, LMC (Author) Other; Quelemes, PV (Author) Other; Lima, DF (Author) Other; Soares, MJS (Author) Other; Pinto, PLS (Author) Other; Mayo, SJ (Author) Other; Ivanova, G (Author) Other; Maria Rangel (Author) ICBAS; Correia, M (Author) Other; Mafud, AC (Author) Other; Mascarenhas, YP (Author) Other; Delerue Matos, C (Author) Other; de Moraes, J (Author) Other; Eaton, P (Author) Other; Leite, JRSA (Author) Other

Journal

Title: Phytotherapy Research

Vol. 31

Pages: 624-630

ISSN: 0951-418X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 27 Citations

Other information

Authenticus ID: P-00M-NJ2

DOI: 10.1002/ptr.5771

Abstract (EN): Pilocarpus microphyllus Stapf ex Wardlew (Rutaceae), popularly known as jaborandi, is a plant native to the northern and northeastern macroregions of Brazil. Several alkaloids from this species have been isolated. There are few reports of antibacterial and anthelmintic activities for these compounds. In this work, we report the antibacterial and anthelmintic activity of five alkaloids found in P.microphyllus leaves, namely, pilosine, epiisopilosine, isopilosine, epiisopiloturine and macaubine. Of these, only anthelmintic activity of one of the compounds has been previously reported. Nuclear magnetic resonance, HPLC and mass spectrometry were combined and used to identify and confirm the structure of the five compounds. As regards the anthelmintic activity, the alkaloids were studied using in vitro assays to evaluate survival time and damaged teguments for Schistosoma mansoni adult worms. We found epiisopilosine to have anthelmintic activity at very low concentrations (3.125 mu g mL(-1)); at this concentration, it prevented mating, oviposition, reducing motor activity and altered the tegument of these worms. In contrast, none of the alkaloids showed antibacterial activity. Additionally, alkaloids displayed no cytotoxic effect on vero cells. The potent anthelmintic activity of epiisopilosine indicates the potential of this natural compound as an antiparasitic agent. Copyright (c) 2017 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7