Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing

• Type: Another Publication in an International Scientific Journal
• Date: 2018

Authors

Oliveira, LM (Author) Other; Maria Inês Barbosa de Carvalho (Author) FEUP; Nogueira, EM (Author) Other; Tuchin, VV (Author) Other

Journal

Title: Journal of Biophotonics

Vol. 11

Final page: e201700094

ISSN: 1864-063X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 11 Citations

ISI Web of Science

Scopus - 16 Citations

Other information

Authenticus ID: P-00N-5CX

DOI: 10.1002/jbio.201700094

Abstract (EN): Skeletal muscle dispersion and optical clearing (OC) kinetics were studied experimentally to prove the existence of the refractive index (RI) matching mechanism of OC. Sample thickness and collimated transmittance spectra were measured during treatments with glucose (40%) and ethylene glycol (EG; 99%) solutions and used to obtain the time dependence of the RI of tissue fluids based on the proposed theoretical model. Calculated results demonstrated an increase of RI of tissue fluids and consequently proved the occurrence of the RI matching mechanism. The RI increase was observed for the wavelength range between 400 and 1000 nm and for the 2 probing molecules explored. We found that for 30 min treatment with 40% glucose and 99% EG, RI of sarcoplasm plus interstitial fluid was increased at 800 nm from 1.328 to 1.348 and from 1.328 to 1.369, respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12