A simple and rapid method for isolation of caliciviruses from liver of infected rabbits

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Teixeira, L (Author) ICBAS; Marques, RM (Author) Other; AP Águas (Author) ICBAS; ferreira, pg (Author) ICBAS

Journal

Title: Research in Veterinary Science

Vol. 91

Pages: 164-166

ISSN: 0034-5288

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-002-PB2

DOI: 10.1016/j.rvsc.2010.08.004

Abstract (EN): Rabbit Haemorrhagic Disease Virus (RHDV), a member of the Caliciviridae family, is the etiologic agent of Rabbit Haemorrhagic Disease (RHD): this viral disease is highly contagious and kills more than 90% of infected adult rabbits. Research on experimental calicivirus infection uses inocula obtained from livers of rabbits dying from calicivirus infection. This implies that caliciviruses have to be purified from liver homogenates. Current methods to isolate caliciviruses from rabbit livers are time consuming. We propose here a new procedure for fast purification of rabbit caliciviruses from liver homogenates that uses centrifugation through an iodixanol gradient. This method offers in approximately 2 h a sample with a high degree of calicivirus purity, as shown by its biochemical and immunocytochemistry analysis, which is also able to kill adult rabbits from RHD within 48 h of inoculation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3