Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Loureiro, J (Author) Other; Pinto, G (Author) Other; Lopes, T (Author) Other; Dolezel, J (Author) Other; Santos, C (Author) Other

Journal

Title: Planta

Vol. 221

Pages: 815-822

ISSN: 0032-0935

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 70 Citations

Scopus - 79 Citations

Other information

Authenticus ID: P-000-28M

DOI: 10.1007/s00425-005-1492-x

Abstract (EN): Flow cytometry analyses were used to verify the ploidy stability of Quercus suber L. somatic embryogenesis process. Leaf explants of two adult cork oak trees (QsG0 and QsG5) of the North of Portugal were inoculated on MS medium with 2,4-D and zeatin. After 3 months, calluses with embryogenic structures were isolated and transferred to fresh MS medium without growth regulators and somatic embryo evolution was followed. Morphologically normal somatic embryos (with two cotyledons) and abnormal somatic embryos (with one or three cotyledons) were used in this assay. Flow cytometry combined with propidium iodide staining was employed to estimate DNA ploidy levels and nuclear DNA content of somatic embryos and leaves from mother plants. No significant differences (P <= 0.05) were detected among embryos, and between the embryos and the mother plants. Also, after conversion of these embryos, no significant morphological differences were observed among the somatic embryo-derived plants. These results and further studies using converted plantlet leaves and embryogenic callus tissue indicate that embryo cultures and converted plantlets were stable with regard to ploidy level. As no major somaclonal variation was detected our primary goal of "true-to-type" propagation of cork oak using somatic embryogenesis was assured at this level. The estimation of the 2C nuclear DNA content for this species is similar to the previously obtained value.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8