An Insight on Salting-out Assisted Liquid-Liquid Extraction for Phytoanalysis

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Valente, IM (Author) FCUP; Moreira, MM (Author) Other; Neves, P (Author) Other; da Fe, T (Author) Other; Goncalves, LM (Author) Other; Almeida, PJ (Author) FCUP; Rodrigues, JA (Author) FCUP

Journal

Title: Phytochemical Analysis

Vol. 28

Pages: 297-304

ISSN: 0958-0344

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00N-3R7

DOI: 10.1002/pca.2676

Abstract (EN): IntroductionSalting-out assisted liquid-liquid extraction (SALLE) is a technique that, although simple and not requiring any complex equipment, is very powerful and versatile. It has obtained growing interest in bioanalysis particularly when combined with chromatographic techniques. ObjectivesHerein, fennel seeds (Foeniculum vulgare Mill.) were used as a case-study to show the application of SALLE in phytochemical analysis. Material and MethodsSALLE combined with HPLC-UV-MS/MS and GC-MS. ResultsBy HPLC-UV-MS/MS analysis of the organic extract it was possible to identify various phenolic compounds, including quercetin derivatives, caffeic acid, p-coumaric acid and chlorogenic acid. The main compounds identified by GC-MS were estragole, fenchone, anisaldehyde, anethole, benzaldehyde, camphor and apiole. ConclusionHPLC and GC analysis of the extracts showed that it is possible to isolate, in only one step, a wide range of compounds with distinct properties, allowing a detailed phytochemical analysis. Copyright (c) 2017 John Wiley & Sons, Ltd. Salting-out assisted liquid-liquid extraction (SALLE) aiming phytochemical analysis. Around fifty relevant compounds identified by LC-MS and GC-MS. Extraction was performed in one optimized SALLE step at room temperature.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8