Isolation of Cells Specialized in Anticancer Alkaloid Metabolism by Fluorescence-Activated Cell Sorting

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Carqueijeiro, I (Author) Other; Guimaraes, AL (Author) Other; Bettencourt, S (Author) Other; Martinez Cortes, T (Author) Other; Guedes, JG (Author) Other; Gardner, R (Author) Other; Lopes, T (Author) Other; Andrade, C (Author) Other; Bispo, C (Author) Other; Martins, NP (Author) Other; Paula B Andrade (Author) FFUP; Patricia Valentao (Author) FFUP; Valente, IM (Author) FCUP; Rodrigues, JA (Author) FCUP; Duarte, P (Author) Other; Mariana Sottomayor (Author) FCUP

Journal

Title: Plant Physiology

Vol. 171

Pages: 2371-2378

ISSN: 0032-0889

Publisher: American Society of Plant Biologists

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00K-QM4

DOI: 10.1104/pp.16.01028

Abstract (EN): Plant specialized metabolism often presents a complex cell-specific compartmentation essential to accomplish the biosynthesis of valuable plant natural products. Hence, the disclosure and potential manipulation of such pathways may depend on the capacity to isolate and characterize specific cell types. Catharanthus roseus is the source of several medicinal terpenoid indole alkaloids, including the low-level anticancer vinblastine and vincristine, for which the late biosynthetic steps occur in specialized mesophyll cells called idioblasts. Here, the optical, fluorescence, and alkaloid-accumulating properties of C. roseus leaf idioblasts are characterized, and a methodology for the isolation of idioblast protoplasts by fluorescence-activated cell sorting is established, taking advantage of the distinctive autofluorescence of these cells. This achievement represents a crucial step for the development of differential omic strategies leading to the identification of candidate genes putatively involved in the biosynthesis, pathway regulation, and transmembrane transport leading to the anticancer alkaloids from C. roseus.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8