Involvement of endothelium in the vasorelaxant effects of 3,4-DHPEA-EA and 3,4-DHPEA-EDA, two major functional bioactives in olive oil

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Segade, M (Author) Other; Bermejo, R (Author) Other; Silva, A (Author) FCUP; Fatima Paiva Martins (Author) FCUP; Gil Longo, J (Author) Other; Campos Toimil, M (Author) Other

Journal

Title: Journal of Functional Foods

Vol. 23

Pages: 637-646

ISSN: 1756-4646

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-00K-AVY

DOI: 10.1016/j.jff.2016.03.024

Abstract (EN): The olive oil polyphenols 3,4-DHPEA-EA and 3,4-DHPEA-EDA displayed an endothelium dependent vasorelaxant effect in rat aorta, starting at similar to 1 mu M and abolished by N-G-nitro-L-arginine (L-NA) or N-acetylcysteine, and an endothelium-independent vasorelaxant effect, starting at similar to 10 mu M. Hydroxytyrosol only presented an endothelium-independent effect at 100 mu M. DHPEA-EA and 3,4-DHPEA-EDA, but not hydroxytyrosol, also increased NO generation within endothelial cells. At higher concentrations, the three compounds reduced argininevasopressin-induced increase of cytosolic Ca2+ concentration ([Ca2+](c)) in vascular myocytes. By UV-visible spectroscopy, we found that these polyphenols undergo autoxidative processes in organ-bath conditions. Thus, 3,4-DHPEA-EA and 3,4-DHPEA-EDA have an endothelium-dependent vasorelaxant effect caused by an enhanced NO production, probably through a redox mechanism within endothelial cells and an endothelium-independent vasorelaxant effect mediated by a reduction of agonist-induced [Ca2+](c) increase in vascular myocytes. Bearing in mind the plasmatic concentrations of these polyphenols following dietary intake of olive oil, these effects could modulate vascular tone in vivo.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10