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Chromatographic separation of prebiotic oligosaccharides. Case study: Separation of galacto-oligosaccharides on a cation exchanger

Title
Chromatographic separation of prebiotic oligosaccharides. Case study: Separation of galacto-oligosaccharides on a cation exchanger
Type
Article in International Scientific Journal
Year
2014
Authors
Łukasz Wianiewski
(Author)
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Carla S. M. Pereira
(Author)
FEUP
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Milan Polakovic
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Alírio E. Rodrigues
(Author)
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Journal
Title: AdsorptionImported from Authenticus Search for Journal Publications
Vol. 20 No. 2-3
Pages: 483-492
ISSN: 0929-5607
Publisher: Springer Nature
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Authenticus ID: P-009-JF2
Abstract (EN): Chromatographic separation of prebiotic oligosaccharides, galacto-oligosaccharides from a typical post-reaction mixture containing unreacted substrate lactose and by-products glucose and galactose was investigated. A commercial cation-exchange resin Dowex 50WX4 was chosen as a prospective preparative-scale adsorbent and separation performance of its four ionic forms, H+, Na+, K+ and Ca2+, was tested. Since adsorption equilibrium isotherms were linear within the entire investigated range of concentrations, they were determined by pulse experiments for all saccharides present in a typical post reaction mixture including tri- and tetragalacto-oligosaccharides. From the four counter ions listed above, hydrogen offered the best selectivity and column performance. The selected H+-form of the cation exchanger was further investigated by means of frontal analysis where high ionic strength and elevated viscosity appear and can play a substantial role. Breakthrough curves were measured for monosaccharides, lactose, glucose-lactose mixture and a multicomponent commercial mixture of galacto-oligosaccharides, Vivinal® GOS. The breakthrough curves were successfully described by the dispersive plug flow model with linear driving force approximation. © 2013 Springer Science+Business Media New York.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 10
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