In vitro cytotoxicity evaluation of resveratrol-loaded nanoparticles: Focus on the challenges of in vitro methodologies

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Rocha, V (Author) Other; Marques, C (Author) Other; Figueiredo, JL (Author) Other; Rita Gaio (Author) FCUP; Paulo Costa (Author) FFUP; Sousa Lobo JM (Author) FFUP; Almeida, IF (Author) FFUP

Journal

Title: Food and Chemical Toxicology

Vol. 103

Pages: 214-222

ISSN: 0278-6915

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 24 Citations

Other information

Authenticus ID: P-00M-JS4

DOI: 10.1016/j.fct.2017.03.017

Abstract (EN): Assessment of toxic effects is mandatory before market placement of pharmaceutical and cosmetic products. Nanotoxicology is an emerging regulatory concern and still a challenging field. Topical application of resveratrol (RSV) has been extensively studied owing to its multi-mechanistic skin anti-aging effects. Nanoencapsulation has been suggested as a promising solution to overcome RSV stability issues. In this work RSV-loaded solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) were prepared using a homogenization/sonication technique. Cytotoxicity assays were conducted with an immortalized cell line of human keratinocytes (HaCaT). For a comprehensive cytotoxicity characterization MIT and Alamar Blue reduction assays (assessment of metabolic activity), Neutral red uptake (evaluation of lysosomal integrity), and Trypan blue (assessment of membrane integrity) were used. The results obtained with the different assays were not always concordant, as put in evidence by an adequate statistical analysis. Experimental parameters such as washing steps were found to be critical. The study is of interest because it draws attention to the importance of careful selected experimental conditions of in vitro nanotoxicological tests. Experimental protocols should be adapted taking into account nano related features such as interference with the dyes and light dispersion/absorption properties.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9