Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Garcia, J (Author) Other; Oliveira, A (Author) Other; de Pinho, PG (Author) FFUP; Victor de Freitas (Author) FCUP; Carvalho, A (Author) Other; Baptista, P (Author) Other; Pereira, E (Author) Other; Maria de Lourdes Bastos (Author) FFUP; Felix Carvalho (Author) FFUP

Journal

Title: Mycologia

Vol. 107

Pages: 679-687

ISSN: 0027-5514

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 24 Citations

Other information

Authenticus ID: P-00G-RK8

DOI: 10.3852/14-253

Abstract (EN): Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotcodns in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. a-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9