Molecular study of mucin-procyanidin interaction by fluorescence quenching and Saturation Transfer Difference (STD)-NMR

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Brandao, E (Author) Other; Silva, MS (Author) Other; Garcia Estevez, I (Author) Other; Nuno Mateus (Author) FCUP; Victor de Freitas (Author) FCUP; Susana Soares (Author) FCUP

Journal

Title: Food Chemistry

Vol. 228

Pages: 427-434

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 35 Citations

Scopus - 36 Citations

Other information

Authenticus ID: P-00M-F76

DOI: 10.1016/j.foodchem.2017.02.027

Abstract (EN): Astringency is closely related to the interaction between procyanidins and salivary proteins (SP). The aim of this work was to study the interaction between mucin, a SP responsible for saliva lubricating properties, with different procyanidins (B4 dimer, tetramer (TT) and fractions of oligomeric procyanidins), and the influence of several conditions [pH, ionic strength, procyanidins' mean degree of polymerization (mDP) and different solvents (ethanol or dimethylsulfoxide)] on this interaction by fluorescence quenching and Saturation Transfer Difference (STD)-NMR. For fractions of oligomeric procyanidins, the mucin-procyanidin interaction increased with mDP; however, for pure compounds, procyanidin TT has lower affinity than dimer B4 which could be due to a lower structural flexibility imposed by its complex structure. Furthermore, EtOH and DMSO can disrupt the main driving forces of these interactions, hydrophobic interactions and hydrogen bonds, respectively, lowering significantly the binding constants.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8