Callus formation and plant regeneration from protoplasts of sunflower calli and hypocotyls

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Santos, C (Author) Other; Caldeira, G (Author) Other

Journal

Title: Acta Societatis Botanicorum Poloniae

Vol. 67

Pages: 31-36

ISSN: 0001-6977

Publisher: Polskie Towarzystwo Botaniczne/Polish Botanical Society

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-001-8NX

Abstract (EN): Sunflower (cv. Girapac SH222) protoplasts were obtained from 4-7 day-old hypocotyls and cotyledons and from two-month old calli. Higher yields of protoplasts were achieved with medium E1 (KCl 25 g . dm(-3), CaCl2 2 g . dm(-3), MES 0.7 g . dm(-3), pH 5.5) and the combination of Driselase Fluka 0.2%, Macerozyme Onozuka 0.2% and Cellulase Onozuka R10 0.2%. Hypocotyls gave the highest yields of protoplasts, followed by cotyledons and calli. Protoplasts were cultivated in liquid and on solid media using both L4M (Burrus et al., 1991) and V-KM (Bokelman and Roest, 1983) media. Culture on solid M1 medium (L4M medium supplemented with NAA 3.0 mg . dm(-3), 2,4-D 0.1 mg . dm(-3) and BA 1.0 mg . dm(-3)) gave a good planting efficiency with the development of many white-green colonies. These colonies gave rise to small calli which were transferred to MSmod medium (MS medium supplemented with KCl 5 g . dm(-3), and polyvinylpyrrolidone (PVP, 4 g . dm(-3)) containing benziladenine (BA, 0.5 mg . dm(-3)), naphtaleneacetic acid (NAA, 0.5 mg . dm(-3)) and giberelic acid (GA3 0.1 mg . dm(-3)). After two weeks, calli were transferred to MSmod medium containing BA 1.0 mg . dm(-3), NAA 0.1 mg . dm(-3), and GA(3) 0.1 mg . dm(-3) for shoot formation. Shoots were excised and induced to root in MSmod supplemented with BA 0.1 mg . dm(-3), NAA 1.0 mg . dm(-3), and GA(3) 0.1 mg . dm(-3). Plantlets were then transferred to sterilised vermiculite for greenhouse acclimation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6