Micropropagation of the Mediterranean species Viburnum tinus

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Nobre, J (Author) Other; Santos, C (Author) Other; Romano, A (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 60

Pages: 75-78

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 25 Citations

Other information

Authenticus ID: P-001-217

DOI: 10.1023/a:1006405700121

Abstract (EN): In vitro propagation of the Mediterranean species Viburnum tinus L. was established from an outdoor-grown shrub. Two standard macrosalt formulations (Margara N30K and Murashige and Skoog), a range of benzyladenine and sucrose concentrations were tested for their effect on shoot multiplication. The cytokinin concentration was the most important factor affecting shoot multiplication. The highest shoot multiplication rate was obtained from single-node explants cultured on Murashige and Skoog medium supplemented with 4.4 mu M benzyladenine. Cytokinin concentration and an interaction of macrosalts and benzyladenine influenced shoot length on the multiplication stage: best shoot growth was observed on MS medium containing 1.1 mu M benzyladenine. In addition, sucrose concentrations of 87.6-146.0 mM gave the highest multiplication rates and improved shoot growth. Following a shoot ellongation stage, single shoots were rooted on media containing naphtaleneacetic acid (1.3-5.4 mu M). Although enhanced in vitro rooting was obtained on media containing 5.4 mu M naphtaleneacetic acid, reducing the auxin concentration to 1.3 mu M during the in vitro rooting stage improved acclimatisation frequency and further plant growth in a horticultural substrate.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4