Comparative responses of Helianthus annuus plants and calli exposed to NaCl: II. Selection of stable salt tolerant calli cell lines and evaluation of osmotic adjustment and morphogenic capacity

• Type: Article in International Scientific Journal
• Date: 2000

Authors

dos Santos, CLV (Author) Other; Gomes, S (Author) Other; Caldeira, G (Author) Other

Journal

Title: Journal of Plant Physiology

Vol. 156

Pages: 68-74

ISSN: 0176-1617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-001-1T1

Abstract (EN): Salt tolerant cell lines of Helianthus annuus L. cv SH222 were selected by sub-culturing cotyledon-derived calli on MSmod medium containing 0, 50, 100, 200 and 300 mmol/L NaCl. NaCl-adapted calli were compact and developed a dark green colour but sustained a regular growth on salt media. Calli adapted to 200 mmol/L (HAZ) and to 300 mmol/L NaCl (HA3) had maximum growth rates at, respectively, 50 and 100 mmol/L NaCl; Na, Cl- and B contents increased in HA2 and HA3 calli, whereas K, Ca, NO3-, HPO42-, Co, Fe and Mo levels decreased. There was an increase in glucose, fructose and sucrose levels in NaCl-adapted calli. Also, the contents of free amino acids increased significantly in adapted calli. This increase was mainly due to Pro followed by Set and Gly. The presence of 50 mmol/L NaCl increased the rate of shoot formation, while higher concentrations inhibited it. Rooting was completely inhibited even at low concentrations of NaCl. Leaves From tolerant shoots (HA2-2 and HA2-5 lines) were transferred to MSmod medium to induce calli in the absence of NaCl. Calli from HA2-5 line sustained growth after transference to 200 mmol/L NaCl, indicating the persistence of salt tolerance at the mitotic level. It was possible to regenerate plants from HA2-5 shoots but plants were stunted and did not survive to acclimation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7