Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Leal, F (Author) Other; Loureiro, J (Author) Other; Rodriguez, E (Author) Other; Pais, MS (Author) Other; Santos, C (Author) Other; Pinto Carnide, O (Author) Other

Journal

Title: Plant Cell Reports

Vol. 25

Pages: 978-985

ISSN: 0721-7714

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 39 Citations

Other information

Authenticus ID: P-004-HQP

DOI: 10.1007/s00299-006-0162-1

Abstract (EN): Flow cytometry was employed to determine the ploidy level of Vitis vinifera L. somatic embryo-derived plants obtained from anther culture. Only one among the 41 analysed plants (2.4%) presented somaclonal variation (tetraploidy); the other plants were diploid. No significant differences (P <= 0.05) were detected between diploid and parental field plants. No haploid or aneuploid plants were observed. The nuclear DNA content of nine V. vinifera cultivars was also estimated using flow cytometry. A non-significant variation was found among the cultivars, with DNA content ranging from 1.17 pg/2C (cv. 'Tinta Barroca' and 'Viosinho') to 1.26 pg/2C (cv. 'Cabernet Sauvignon'). These results and previous studies on other Vitis species suggest that Vitis genome is stable with regard to nuclear DNA content.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8