Factors affecting maintenance, proliferation, and germination of secondary somatic embryos of Eucalyptus globulus Labill

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Pinto, G (Author) Other; Park, YS (Author) Other; Silva, S (Author) Other; Neves, L (Author) Other; Araujo, C (Author) Other; Santos, C (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 95

Pages: 69-78

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 39 Citations

Scopus - 44 Citations

Other information

Authenticus ID: P-003-W0W

DOI: 10.1007/s11240-008-9417-6

Abstract (EN): The described protocol for repetitive somatic embryogenesis (SE) in Eucalyptus globulus produced more somatic embryos than the primary SE protocol. Primary somatic embryos (induced on MS(3NAA)) were transferred to the same medium, leading to new cycles of somatic embryos, for at least 2 years. The influence of medium (MS and B5), plant growth regulators (auxins and cytokinins), and light on secondary SE was tested. The MS medium without growth regulators (MS(WH)) was more efficient for cotyledonary embryo formation and germination than the B5 medium. Reducing auxin (NAA) levels increased the proliferation of globular somatic embryos and allowed SE competence to be maintained on medium free of plant growth regulators. The addition of two cytokinins (BAP and KIN) to the MS medium did not improve proliferation of globular secondary embryos, but was crucial during later stages of the SE process (germination and conversion). Data also show that light may influence the quality of the process, depending on its stage. Darkness should be maintained until the cotyledonary stage is reached, after which exposure to light is recommended.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10