Cadmium-induced genetic instability in mice testis

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Oliveira, H (Author) Other; Lopes, T (Author) Other; Almeida, T (Author) Other; Pereira, MD (Author) Other; Santos, C (Author) Other

Journal

Title: HUMAN & EXPERIMENTAL TOXICOLOGY

Vol. 31

Pages: 1228-1236

ISSN: 0960-3271

Publisher: SAGE Publications

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 27 Citations

Other information

Authenticus ID: P-002-2XB

DOI: 10.1177/0960327112445937

Abstract (EN): Cadmium is a well recognized carcinogenic, cytotoxic and mutagenic transition metal. Recent evidence suggests that the proteins participating in the DNA repair systems, especially in excision and mismatch repair (MMR), are sensitive targets of cadmium toxicity. Microsatellite instability (MSI) is regarded as one of the phenotypes of defective DNA MMR and, consequently, as a marker of high risk for cancer. The purpose of this work is to determine whether cadmium, in the form of cadmium chloride (CdCl2), may induce microsatellite mutations in murine testes. For this study, 2-month-old male ICR-CDI mice were treated by a single subcutaneous injection of I, 2 and 3 mg CdCl2/kg body weight and killed after 35 days. A panel of six microsatellite markers, previously reported as being the most sensitive in detecting MSI in murine tumours, was used in this study. The results show that CdCl2 in the doses of 2 and 3 mg/kg induced a decrease in the testis weight and severe histopathologic changes with complete disorganization of testicular structure and evidences of severe necrosis. In addition, the animals exposed to the lowest CdCl2 dose presented MSI in the testis. The results indicate the existence of MSI in at least two nuclear loci suggesting putative genotoxic effects induced by cadmium.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9