Cadmium-induced genotoxicity in human osteoblast-like cells

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Oliveira, H (Author) Other; Monteiro, C (Author) Other; Pinho, F (Author) Other; Pinho, S (Author) Other; Ferreira de Oliveira, JMPF (Author) Other; Santos, C (Author) Other

Journal

Title: Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Vol. 775

Pages: 38-47

ISSN: 1383-5718

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 31 Citations

Other information

Authenticus ID: P-009-ZZC

DOI: 10.1016/j.mrgentox.2014.10.002

Abstract (EN): Cadmium (Cd) is a widespread heavy metal used in numerous industrial processes. Cd exerts toxicological effects mostly in kidney and liver. Bone is also an important target of Cd, however, the cellular mechanisms of Cd toxicological effects in the bone cells are still poorly understood. Therefore, the present work aimed to investigate the putative cytotoxic and genotoxic effects of Cd to human bone cells. For that, the osteoblast-like MG-63 cells were exposed to 20 and 50 mu M Cd for 24 and 48 h. Results showed a dose-dependent increase in Cd accumulation in cells and a decrease in cell viability, especially after 48 h. Cell cycle analysis showed a delay at S phase concomitant with a decrease in cells at G0/G1 phase. After 24 h, Cd treatment downregulated the expression of CHEK1, CHEK2 and CDK2 genes and upregulated the expression of CCNE1 gene. After 48 h, the expression of ATM and CCNB1 genes were downregulated. Also, a 3.3 fold increase on the expression of gene CCNE1 was detected. Both Cd doses induced DNA fragmentation at 48 h, while an increase in micronuclei (MN) and nucleoplasmic bridges (NPBs) together with an increase in the percentage of apoptotic/necrotic cells was detected for both time periods. Overall, our results demonstrate the cytotoxicity and genotoxicity of Cd in human bone cells. Also, the cytokinesis-block micronucleus (CBMN) assay parameters (MN, NPBs and the percentage of cells under apoptosis or necrosis) together with the cell cycle appear as the most sensitive to Cd cyto- and genotoxicity, being early affected even with the lowest Cd dose. Therefore, these cyto-/genotoxic techniques may be selected for early detection of Cd-induced toxicity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10