A study of the effects of citrate-coated silver nanoparticles on RAW 264.7 cells using a toolbox of cytotoxic endpoints

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Bastos, V (Author) Other; Duarte, IF (Author) Other; Santos, C (Author) FCUP; Oliveira, H (Author) Other

Journal

Title: Journal of Nanoparticle Research

Vol. 19

ISSN: 1388-0764

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00M-RKZ

DOI: 10.1007/s11051-017-3855-1

Abstract (EN): Citrate-coated silver nanoparticles (citrate-AgNPs) are among the most commonly used nanomaterials, widely present in industrial and biomedical products. In this study, the cytotoxicity of 30-nm citrate-AgNPs on the macrophage cell line RAW 264.7 was evaluated, using a battery of cytotoxicity endpoints (viability, oxidative stress, and cytostaticity/clastogenicity), at 24 and 48 h of exposure. Citrate-AgNPs decreased cell proliferation and viability only at 75 mu g/mL, suggesting a low sensitivity of RAW cells to lower doses of these AgNPs. After 24 h of exposure, ROS content decreased in cells exposed to 60 mu g/mL AgNPs (IC20 value), corroborating the high tolerance of these cells to citrateAgNPs. However, these cells suffered an impairment of the cell cycle, shown by an increase at the sub-G1 phase. This increase of the sub-G1 population was correlated with an increase of DNA fragmentation, suggesting an increase of apoptosis. Thus, our data are important to understand the effects of low concentrations (IC20) of citrate-AgNPs on in vitro vital macrophage functions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9