Pb low doses induced genotoxicity in Lactuca sativa plants

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Silva, S (Author) Other; Silva, P (Author) Other; Oliveira, H (Author) Other; Gaivao, I (Author) Other; Matos, M (Author) Other; Pinto Carnide, O (Author) Other; Santos, C (Author) FCUP

Journal

Title: Plant Physiology and Biochemistry

Vol. 112

Pages: 109-116

ISSN: 0981-9428

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 27 Citations

Other information

Authenticus ID: P-00M-K5A

DOI: 10.1016/j.plaphy.2016.12.026

Abstract (EN): Soil and water contamination by lead (Pb) remains a topic of great concern, particularly regarding crop production. The admissible Pb values in irrigation water in several countries range from approximate to 0.1 to approximate to 5 mg L-1. In order to evaluate putative effects of Pb within legal doses on crops growth, we exposed Lactuca sativa seeds and seedlings to increasing doses of Pb(NO3)(2) up to 20 mg L-1. The OECD parameter seed germination and seedling/plant growth were not affected by any of the Pb-concentrations used. However, for doses higher than 5 mg L-1 significant DNA damage was detected: Comet assay detected DNA fragmentation at >= 5 mg L-1 and presence of micronuclei (MN) were detected for 20 mg L-1 Also, cell cycle impairment was observed for doses as low as 0.05 mg L-1 and 0.5 mg L-1 (mostly G(2) arrest). Our data show that for the low doses of Pb used, the OECD endpoints were not able to detect toxicity, while more sensitive endpoints (related with DNA damage and mitotic/interphase disorders) identified genotoxic and cytostatic effects. Furthermore, the nature of the genotoxic effect was dependent on the concentration. Finally, we recommend that MN test and the comet assay should be included as sensitive endpoints in (eco)toxicological assays.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8