Evaluation of polysomaty and estimation of genome size in Polygala vayredae and P-calcarea using flow cytometry

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Castro, S (Author) Other; Loureiro, J (Author) Other; Rodriguez, E (Author) Other; Silveira, P (Author) Other; Navarro, L (Author) Other; Santos, C (Author) Other

Journal

Title: Plant Science

Vol. 172

Pages: 1131-1137

ISSN: 0168-9452

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-004-9Y8

DOI: 10.1016/j.plantsci.2007.03.002

Abstract (EN): In seed plants, endopolyploidy is regarded as a common and developmentally regulated phenomenon. However, in Polygalaceae polysomaty has never been studied. In this work the endopolyploidy of Polygala vayredae (2n = 28, subgenus Chamaebuxus) and P. calcarea (2n = 34, subgenus Poly-gala) was evaluated using flow cytometry. With this technique it was possible to observe polysomaty in endosperm, leaves and petals of both species, although with different patterns. Usually, in P. vayredae, 2C and 4C ploidy levels were detected while for leaves of P. calcarea, an extra 8C level was observed. In P. vayredae, statistically significant differences were observed in the endopolyploid level between fully expanded young leaves and 1-year-old mature leaves. Nuclear DNA content analysis in these taxa revealed significant differences, with P vayredae presenting a higher genome size (2C = 2.71 pg DNA) than P. calcarea (2C = 0.98 pg DNA). These data and the highest level of polysomaty found in P. calcarea seem to point to a negative correlation between genome size and endopolyploidy, as observed in other works.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7