A protocol for Ulmus minor Mill. micropropagation and acclimatization

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Conde, P (Author) Other; Sousa, A (Author) Other; Costa, A (Author) Other; Santos, C (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 92

Pages: 113-119

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 32 Citations

Other information

Authenticus ID: P-004-4EM

DOI: 10.1007/s11240-007-9310-8

Abstract (EN): Here we report the establishment of a simple protocol for the micropropagation and acclimatization of U. minor. Branches with dormant buds were collected from mature elms and sprouted in a greenhouse. Tip and node segments were used as starting material for in vitro proliferation in a medium (designated here as DKW1) already used for the micropropagation of a clone of the English Elm (U. procera SR4). In the first assay, in which explants from nine different trees were used, 88.5% of the tip segments produced new axillary shoots thus proving to be the best explant type. Afterwards, material from four different trees (F4, F7, F13, F14), that had the highest sprouting rate in the greenhouse, was used to test for genotype influence. F14 proved to be the best genotype in culture and it was used for all the subsequent experiments. Shoots from F14 were used to assay in vitro rooting using five DKW based media. Rooting percentages were high for all media and varied between 80% and 100%. For acclimatization two approaches were assayed: the use of previously rooted in vitro plants and the direct acclimatization of shoots from cultures in DKW1. After 6 weeks, 86.4% of the in vitro rooted plants were successfully acclimatized and a slightly higher value, 88.6%, was attained by direct acclimatization of shoots with thick stems and hard leaves. These results proved that there is no need for a previous in vitro rooting step and that direct acclimatization can effectively reduce time and costs. Thus U. minor micropropagation and acclimatization can be divided into only two steps: proliferation of shoots in DKW1 and direct acclimatization of these shoots in a sterile soil mixture.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7