Supercritical CO2 assisted process for the production of high-purity and sterile nano-hydroxyapatite/chitosan hybrid scaffolds

• Type: Article in International Scientific Journal
• Date: 2018-04

Authors

G. Ruphuy (Author) Other; M. Souto Lopes (Author) Other; Diana Paiva (Author) FEUP; P. Costa (Author) Other; Alírio E. Rodrigues (Author) FEUP; Fernando Mendes Monteiro (Author) FEUP; C. L. Salgado (Author) Other; Maria Helena Fernandes (Author) Other; José Carlos B. Lopes (Author) FEUP; Madalena M. Dias (Author) FEUP; M. F. Barreiro (Author) Other

Journal

Title: Journal of Biomedical Materials Research - Part B Applied Biomaterials

Vol. 106 No. 101B

Pages: 965-975

ISSN: 1552-4973

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 9 Citations

ISI Web of Science - 5 Citations

Scopus - 10 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

LSRE-LCM - Laboratório de Processos de Separação e Reação - Laboratório de Catálise e Materiais

AIProcMat@N2020 - Advanced Industrial Processes and Materials for a Sustainable North Region of Portugal2020

Other information

Authenticus ID: P-00M-W98

DOI: 10.1002/jbm.b.33903

Abstract (EN): Hybrid scaffolds composed of hydroxyapatite (HAp), in particular in its nanometric form (n-HAp), and chitosan (CS) are promising materials for non-load-bearing bone graft applications. The main constraints of their production concern the successful implementation of the final purification/neutralization and sterilization steps. Often, the used purification strategies can compromise scaffold structural features, and conventional sterilization techniques can result in material's thermal degradation and/or contamination with toxic residues. In this context, this work presents a process to produce n-HAp/CS scaffolds mimicking bone composition and structure, where an innovative single step based on supercritical CO2 extraction was used for both purification and sterilization. A removal of 80% of the residual acetic acid was obtained (T=75 degrees C, p=8.0 MPa, 2 extraction cycles of 2 h) giving rise to scaffolds exhibiting adequate interconnected porous structure, fast swelling and storage modulus compatible with non-load-bearing applications. Moreover, the obtained scaffolds showed cytocompatibility and osteoconductivity without further need of disinfection/sterilization procedures. Among the main advantages, the proposed process comprises only three steps (n-HAp/CS dispersion preparation; freeze-drying; and supercritical CO2 extraction), and the supercritical CO2 extraction show clear advantages over currently used procedures based on neutralization steps. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 965-975, 2018.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11