A FIA-enzymatic method for the determination of L(+) lactate: application in parenteral solution analysis

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Barrado, E (Author) Other; Vela, MH (Author) Other; Lapa, RAS (Author) FFUP; Lima, JLFC (Author) FFUP

Journal

Title: QUIMICA ANALITICA

Vol. 19

Pages: 241-246

ISSN: 0212-0569

Publisher: Springer

Indexing

ISI Web of Knowledge - 1 Citation

Other information

Authenticus ID: P-001-25P

Abstract (EN): A flow injection system for the determination of L(+)lactate in parenteral solutions was developed. The system is based on the amperometric detection of hydrogen peroxide, produced when lactate is converted to pyruvate by the enzyme lactate oxidase over a platinum electrode. The flow injection manifold contains a dialysis unit to maintain the enzyme in a closed re-circulation loop. Determinations are made without the need to pre-treat the sample. Under optimal conditions of pH, temperature and hydrodynamic and geometric variables obtained by means of a L-9(3(4)) Taguchi parameter design, a linear response was achieved for lactate concentrations within the range 2 to 30 mM, working at 37 degreesC. Reproducibility was good (RSD < 5%, n = 20) at a sampling rate of 25 determinations/hour. The results obtained were in close agreement with those of a reference method (European Pharmacopeia).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6