Dysregulated expression of microRNA-150 in human papillomavirus-induced lesions of K14-HPV16 transgenic mice

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Joana M. O. Santos (Author) Other; Mara Fernandes (Author) Other; Rita Araújo (Author) Other; Hugo Sousa (Author) Other; Joana Ribeiro (Author) Other; Margarida M. S. M. Bastos (Author) FEUP; Paula A. Oliveira (Author) Other; Diogo Carmo (Author) Other; Fátima Casaca (Author) Other; Sandra Silva (Author) Other; Ana L. Teixeira (Author) Other; Rui M. Gil da Costa (Author) FEUP; Rui Medeiros (Author) Other

Journal

Title: Life Sciences

Vol. 175

Pages: 31-36

ISSN: 0024-3205

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 12 Citations

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00M-MXV

DOI: 10.1016/j.lfs.2017.03.008

Abstract (EN): Aims: High-risk human papillomavirus (HPV) infection is one of the major causes of infection-related cancers worldwide. MicroRNAs (miRNAs) are a family of non-coding RNAs (ncRNAs), whose dysregulated levels may cause an aberrant expression of genes involved in oncogenic pathways and consequently lead to cancer development. This is the case of the miRNA-150 (miR-150), whose expression in HPV-induced lesions remains unclear and the present work aims to clarify it. We employed K14-HPV16 mice, which express the early genes of HPV16 in basal keratinocytes, leading to the development of hyperplastic and dysplastic skin lesions and squamous cell carcinomas, and are a representative model of HPV-induced cancers. Main methods: In order to evaluate the expression of miR-150 in HPV-induced lesions, we performed qPCR in wild-type mice (HPV-/-) and in skin lesions of K14-HPV16 transgenic mice (HPV+/-). Matched skin samples were analyzed histologically. Key findings: 24-26 weeks-old HPV+/- mice showed diffuse epidermal hyperplasia and focal dysplasia in a hyperplastic background (31.8% incidence), but 28-30 weeks-old HPV+/- mice presented higher incidence of dysplasia (100.0%). MiR-150 was upregulated in HPV-/- mice when compared with HPV-/- mice (p < 0.001). MiR150 was also overexpressed in diffuse dysplastic lesions when compared with hyperplastic lesions (p = 0.005). Significance: The present results suggest that miR-150 is overexpressed in HPV-induced lesions in this model and its expression seems to increase with lesion progression, along the process of multi-step carcinogenesis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6