Study of the suitability of 2-(N-morpholino) ethanesulfonic acid pH buffer for heavy metals accumulation studies using Saccharomyces cerevisiae

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Eduardo V. Soares (Author) Other; Andreia P. R. S. Duarte (Author) Other; Helena Soares (Author) FEUP

Journal

Title: Chemical Speciation and Bioavailability

Vol. 12 No. 2

Pages: 59-65

ISSN: 0954-2299

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-001-18Z

DOI: 10.3184/095422900782775535

Abstract (EN): Yeast cells of Saccharomyces cerevisiae maintained viability, and no detectable amount of proteins and inorganic phosphate were determined in the filtrate, when incubated during 48 hours in 10 mM of 2-(N-morpholino) ethanesulfonic acid (MES) at pH 6.0. Ultraviolet light-absorbing materials (at 260 nm) increased between 2 and 6 hours (or 24 hours, depending on the strain) of incubation and decreased after this period. The possibility of using MES pH buffer for in vitro heavy metals accumulation studies is discussed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7