Altered expression of CKs 14/20 is an early event in a rat model of multistep bladder carcinogenesis

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Rui M. Gil da Costa (Author) FEUP; Paula A. Oliveira (Author) Other; Carmen Vasconcelos Nóbrega (Author) Other; Regina Arantes Rodrigues (Author) Other; Rosário Pinto Leite (Author) Other; Aura A. Colaço (Author) Other; Luis F. de la Cruz (Author) Other; Carlos Lopes (Author) Other

Journal

Title: International Journal of Experimental Pathology

Vol. 96 No. 5

Pages: 319-325

ISSN: 0959-9673

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Associated Projects

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00J-ZBS

DOI: 10.1111/iep.12145

Abstract (EN): Cytokeratins (CKs) 14 and 20 are promising markers for diagnosing urothelial lesions and for studying their prognosis and histogenesis. This work aimed to study the immunohistochemical staining patterns of CK14/20 during multistep carcinogenesis leading to papillary bladder cancer in a rat model. Thirty female Fischer 344 rats were divided into three groups: group 1 (control); group 2, which received N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) for 20 weeks plus 1 week without treatment; and group 3, which received BBN for 20 weeks plus 8 weeks without treatment. Bladder lesions were classified histologically. CK14 and CK20 immunostaining was assessed according to its distribution and intensity. In control animals, 0-25% of basal cells and umbrella cells stained positive for CK14 and CK20 respectively. On groups 2 and 3, nodular hyperplastic lesions showed normal CK20 and moderately increased CK14 staining (26-50% of cells). Dysplasia, squamous metaplasia, papilloma, papillary tumours of low malignant potential and low- and high-grade papillary carcinomas showed increased CK14 and CK20 immunostaining in all epithelial layers. Altered CK14 and CK20 expression is an early event in urothelial carcinogenesis and is present in a wide spectrum of urothelial superficial neoplastic and preneoplastic lesions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7