Cytogenetic characterization of an N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse papillary urothelial carcinoma

• Type: Article in International Scientific Journal
• Date: 2013

Authors

R. Arantes Rodrigues (Author) Other; R. Pinto Leite (Author) Other; R. M. Gil da Costa (Author) FEUP; A. Colaco (Author) Other; C. Lopes (Author) Other; P. Oliveira (Author) Other

Journal

Title: Tumor Biology

Vol. 34 No. 5

Pages: 2691-2696

ISSN: 1010-4283

Publisher: SAGE

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Associated Projects

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Other information

Authenticus ID: P-006-6XR

DOI: 10.1007/s13277-013-0820-1

Abstract (EN): Chemically-induced urinary bladder cancer in rodents has long been used as a reliable model to study the biopathology of urinary bladder neoplasia and to develop therapeutic strategies against human tumors. Knowledge of the genetic basis underlying carcinogenesis would greatly enhance usability and usefulness of this model for the purposes of comparative pathology. However, little is known about the cytogenetic characteristics of rodent urinary bladder tumors. Accordingly, pathological and negative control specimens were collected for cytogenetic evaluation, from an ongoing mouse urinary bladder N-butyl-N-(4-hydroxybutyl) nitrosamine-induced carcinogenesis study. Histopathological analysis characterized the pathological sample as a papillary urothelial carcinoma. Conventional cytogenetic analysis revealed the presence of 66.3 % tetraploid cells. Fluorescent in situ hybridization using chromosome paint probes allowed the detection of a reciprocal translocation involving chromosomes 4 and 14 (containing the murine homologues to human p16 and retinoblastoma tumor-suppressor genes) in 42 % of tetraploid cells. The control sample showed no histological or cytogenetic changes. CDKN2A and RB1 loss of heterozygosity is associated with human early and advanced urinary bladder cancer, respectively. Thus, the present data paves the way for further studies concerning the molecular mechanisms of urinary bladder carcinogenesis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6