Paired related homeobox protein-like 1 (Prrxl1) controls its own expression by a transcriptional autorepression mechanism

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Monteiro, CB (Author) Other; Costa, MF (Author) Other; reguenga, c (Author) FMUP; Lima, D (Author) Other; Castro, DS (Author) Other; Filipe Monteiro (Author) FMUP

Journal

Title: FEBS Letters

Vol. 588

Pages: 3475-3482

ISSN: 0014-5793

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-009-T2H

DOI: 10.1016/j.febslet.2014.08.006

Abstract (EN): The homeodomain factor paired related homeobox protein-like 1 (Prrxl1) is crucial for proper assembly of dorsal root ganglia (DRG)-dorsal spinal cord (SC) pain-sensing circuit. By performing chromatin immunoprecipitation with either embryonic DRG or dorsal SC, we identified two evolutionarily conserved regions (i.e. proximal promoter and intron 4) of Prrxl1 locus that show tissue-specific binding of Prrxl1. Transcriptional assays confirm the identified regions can mediate repression by Prrxl1, while gain-of-function studies in Prrxl1 expressing ND7/23 cells indicate Prrxl1 can down-regulate its own expression. Altogether, our results suggest that Prrxl1 uses distinct regulatory regions to repress its own expression in DRG and dorsal SC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8