Comprehensive Analysis of Secreted Protein, Acidic and Rich in Cysteine in Prostate Carcinogenesis: Development of a 3D Nanostructured Bone-Like Model

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Ribeiro, N (Author) Other; Costa Pinheiro, P (Author) Other; Henrique, R (Author) Other; Gomez Lazaro, M (Author) Other; Pereira, MP (Author) Other; Mansur, AAP (Author) Other; Mansur, HS (Author) Other; Carmen Jeronimo (Author) ICBAS; Sousa, SR (Author) Other; Fernando Mendes Monteiro (Author) FEUP

Journal

Title: Journal of Biomedical Nanotechnology

Vol. 12

Pages: 1667-1678

ISSN: 1550-7033

Publisher: American Scientific Publishers

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00K-SXT

DOI: 10.1166/jbn.2016.2276

Abstract (EN): Most aggressive prostate cancer (PCa) types tend to metastasize frequently to bone and SPARC, a matricellular protein, might participate in such biological processes. The objective of this study was to evaluate the effect of SPARC in prostate carcinogenesis and bone metastization. This was explored assessing the morphology, metabolic activity and SPARC expression of different PCa cell lines resembling different stages of carcinogenesis, using a 3D bone-biomimetic model (collagen nanofibers/nanohydroxyapatite) grafted with SPARC. Our findings highlight distinct cellular behavior depending on cell type and presence of exogenous SPARC. In fact, SPARC addition contributed to the survival and significant growth of a non-bone metastatic PCa cell line (LNCaP) on bone-like biomaterial. Moreover, SPARC expression levels were evaluated in a series of prostatic tissues, comparing normal prostate, pre-neoplastic prostate intraepithelial neoplasias and overtly malignant tumors, and also metastasis to its correspondent primary prostate tumors, ascertaining potential association between SPARC and clinicopathological data. Remarkably, SPARC was overexpressed in patients with higher Gleason Score, indicating tumors with poor prognosis, as well as in metastasis, particularly from bone sites, compared with their respective primary tumors. The results suggest a potential role of SPARC as a clinical target on PCa, due to its association with bone metastization.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12