Prediction of melting temperatures in fluorescencein situhybridization (FISH) procedures using thermodynamic models

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Sílvia Fontenete (Author) FEUP; Nuno Guimarães (Author) FEUP; Jesper Wengel (Author) Other; Nuno Filipe azevedo (Author) FEUP

Journal

Title: Critical Reviews in Biotechnology

Vol. 36 No. 3

Pages: 566-577

ISSN: 0738-8551

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 19 Citations

Associated Projects

DNA mimics - Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos

LEPAE/CEFT - RL2 - (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00M-GGD

DOI: 10.3109/07388551.2014.993589

Abstract (EN): The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA thermodynamics that provide reasonably accurate thermodynamic information on nucleic acid duplexes and allow estimation of the melting temperature. Because there are no thermodynamic models specifically developed to predict the hybridization temperature of a probe used in a fluorescence in situ hybridization (FISH) procedure, the melting temperature is used as a reference, together with corrections for certain compounds that are used during FISH. However, the quantitative relation between melting and experimental FISH temperatures is poorly described. In this review, various models used to predict the melting temperature for rRNA targets, for DNA oligonucleotides and for nucleic acid mimics (chemically modified oligonucleotides), will be addressed in detail, together with a critical assessment of how this information should be used in FISH.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12