Resumo (PT):
Abstract (EN):
Saccharomyces cerevisiae (S. cerevisiae) is a crucial fermenting microorganism for the beer, wine and
bread industry. As such, an accurate and rapid method for its identification and monitoring is required. In
here, a peptide nucleic acid (PNA) probe was first designed to target S. cerevisiae by fluorescence in situ
hybridization (FISH). This PNA-FISH method was then systematically optimized, employing response
surface methodology (RSM). The interaction between the critical hybridization temperature, time and
formamide concentration and their effect on the FISH efficiency were modelled. The model predicted
optimal fluorescence intensity upon hybridization at 53.9 C, during 57.8 min and using 43.8% (v/v)
formamide in the hybridization buffer, which was experimentally confirmed. RSM showed to be a
valuable tool to optimize and better understand the dynamics of yeast FISH, which can impact the
performance evaluation of related fermentation processes.
Language:
English
Type (Professor's evaluation):
Scientific
No. of pages:
5