Development of an Analytical Methodology for Simultaneous Determination of Vincristine and Doxorubicin in Pharmaceutical Preparations for Oncology by HPLC-UV

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Rodrigues, AS (Author) Other; Lopes, AR (Author) Other; Leao, A (Author) Other; Couceiro, A (Author) Other; Sarmento Ribeiro, ABS (Author) Other; Ramos, F (Author) Other; Noronha da Silveira, MIN (Author) Other; de Oliveira, CR (Author) Other

Journal

Title: Journal of Chromatographic Science

Vol. 47 No. 2

Pages: 387-391

ISSN: 0021-9665

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-003-KEJ

Abstract (EN): A high-performance liquid chromatography-UV methodology (λ= 230 nm) was developed and validated for the simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations used in oncology. The chromatography was carried out on a C18 column using acetonitrile 90% in water-potassium hydrogenphosphate buffer 50mM, pH 3.2 ± 0.1 (32:68, v/v) as mobile phase at a flow rate of 1.5 mL/min. The method proved to be specific, exact, and accurate, in accordance with the ICH standards, presenting linearity in the 1-5 οg/mL and 5-100 οg/mL intervals, and detection (0.19 ×0.51 οg/mL) and quantification (0.63 ×1.7 οg/mL) limits for vincristine and doxorubicin, respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5